Klin Farmakol Farm. 2016;30(1):5-10 | DOI: 10.36290/far.2016.002

The use of goldenrod general (Solidago virgaurea) preparations does not influence the metabolism of concomitantly administered drugs

Veronika Tománková¹, Adéla Vlčková¹, Pavel Anzenbacher², Petr Bachleda³, Eva Anzenbacherová¹

¹ Ústav lékařské chemie a biochemie, Lékařská fakulta Univerzity Palackého, Olomouc

² Ústav farmakologie, Lékařská fakulta Univerzity Palackého, Olomouc

³ II. chirurgická klinika, Fakultní nemocnice Olomouc

Purpose: Solidago virgaurea „goldenrod“ is a known medicinal plant that has been used since ancient times (1, 2). The genus Solidago

is interesting in terms of both biological effects and the presence of many interesting secondary metabolites – flavonoids,

phenolic acids and glucosides, polysaccharides and others (3). Solidago virgaurea is known to exhibit diuretic, antiinflammatory

and newly described antitumoral, antimicrobial, sedative and hypotensive effects (1, 2). Primarily flavonoids and phenolic diglucoside

– leiocarposide belong to secondary metabolites having primarily a positive effect on diuresis (3). Our objective was to

investigate whether Solidaginis virgaureae herba extract, decoction and leachate affect the properties of phase I biotransformation

enzymes, namely cytochromes P450 in human liver microsomal fraction and also in primary cultures of human hepatocytes.

Methods: Different concentrations of Solidaginis virgaureae herba extract, decoction and leachate, expressed as mg of dry weight

in mL of water (0.042 mg/mL; 0.083 mg/mL and 0.167 mg/mL) were studied. Enzyme activities and protein expression were determined

using HPLC and Western blotting, respectively.

Results: Activity and protein expression of CYP1A2, CYP2A6, CYP2C9, CYP2D6 and CYP3A4 were only little influenced by Solidaginis

virgaureae herba preparations, either in human hepatocytes as well as in human liver microsomal fraction in all studied

concentrations.

Conclusion: Based on the results of the enzyme activities and the amount of protein can be expected that the Solidaginis virgaureae

herba preparations do not significantly affect the metabolism of concomitantly administered drugs and their use probably

does not result in drug interactions.

Keywords: Solidaginis virgaureae herba, cytochromes P450, primary cultures of human hepatocytes, human liver microsomal fraction, drug metabolism

Published: May 30, 2016  Show citation